In this section, we will present the certified results obtained through laboratory tests when we applied an aqueous colloidal solution of ozone nanobubbles, produced by our equipment, to different colonies of bacteria and fungus.
It is important to note that all the results obtained in the laboratory are managed by a quality management system certified according to ISO 9001:2015 and accredited according to ISO/IEC 17025:2017, as well as formally accredited by the Brazilian federal government, guaranteeing the veracity and reliability of the results presented below.
Table.1 shows the initial and final biological load values for each type of fungus and bacteria in the tests performed. It is important to note that in the tests, the procedure used consisted of applying the aqueous colloidal solution with the ozone nanobubbles to the colonies only once (1x time) and taking consecutive measurements to analyze the actual reduction of the colonies of sprayed bacteria and fungus. The concentration of ozone nanobubbles in the tests performed was 5 ppm, or, for the test conditions, 4.98 g/m3 of Ozone (O3).
*Tests conducted according to standard protocols:
– Culture plates for fungal analysis: Seeding on 4% Sabouraud Dextrose Agar.
– Culture plates for bacterial analysis: Seeding on enriched, selective and differential Agar: Tryptic Soy Agar (TSA).
Based on the data presented in Table 1, it is possible to observe the high efficiency in combating and reducing the biological loads tested by up to 98.2%, with the lowest reduction rate being 82.9%, i.e. the aqueous colloidal solution of ozone nano-bubbles generated by our technology is highly efficient in combating various pathogens and microorganisms.
Besides that, table.2 shows the percentage reduction of biological charges over time after spraying the solution in time 0 minutes to 60 minutes, i.e., it shows an analysis on the efficiency of ozone colloid in relation to exposure time.
*Tests conducted according to standard protocols:
– Culture plates for fungal analysis: Seeding on 4% Sabouraud Dextrose Agar.
– Culture plates for bacterial analysis: Seeding on enriched, selective and differential Agar: Tryptic Soy Agar (TSA).
Conclusion
Based on the above, we can present some additional considerations on the use of ozone, such as:
- Effectiveness: Ozone is a powerful oxidizing agent capable of eliminating microorganisms, including bacteria, viruses, and fungus, making it ideal for safe, inexpensive disinfection that is free of hazardous residues or harmful to human and animal health.
- Safety: Although effective, the use of ozone must be monitored to avoid inhalation at high concentrations, as it can be toxic indoors.
- Applications: Used in various industries, including food and water and wastewater treatment, as an environmentally friendly alternative to chemical disinfectants.
Sanitation standards in different regions reflect a strict approach to ensuring public safety. The use of ozone as a sanitizing agent is widely recognized and regulated, although implementation and supervision vary among countries. This comparison can help identify best practices and areas for development.
The application of ozone in sanitization stands out in the Italian and global markets for its cost, effectiveness, safety and sustainability characteristics. The case studies presented, along with certification from a respected laboratory accredited by the Brazilian government, demonstrate that this technology not only meets but exceeds expectations in terms of disinfection and infection control, while fully meeting environmental sustainability and cost containment goals. Continued research and adoption of innovative solutions such as ozone are essential to the evolution of sanitization practices globally.